1U28

R. rubrum transhydrogenase asymmetric complex (dI.NAD+)2(dIII.NADP+)1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	71.663, 73.960, 204.171
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.300
Rwork	0.241
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzz
RMSD bond angle	1.164
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	TNT
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.000
High resolution limit [Å]	2.300
Number of reflections	47505
<I/σ(I)>	11.6
Completeness [%]	96.7
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	PEG-8K, AMMONIUM SULPHATE, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K