1U27
Triglycine variant of the ARNO Pleckstrin Homology Domain in complex with Ins(1,3,4,5)P4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2002-07-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.917, 56.024, 59.533 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.300 |
R-factor | 0.23425 |
Rwork | 0.231 |
R-free | 0.28840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fgy |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 5810 | |
Completeness [%] | 88.1 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 4000, sodium MES, 10% glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |