1U22
A. thaliana cobalamine independent methionine synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-09 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979667 |
Spacegroup name | P 65 |
Unit cell lengths | 122.423, 122.423, 131.365 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.780 - 2.650 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.27000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1u1h |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.810 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 32422 | |
<I/σ(I)> | 23 | 4.93 |
Completeness [%] | 99.5 | 97.9 |
Redundancy | 11.2 | 10.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 30% (W/V) POLY-ETHYLENE GLYCOL (PEG) 8000, 0.2 M AMMONIUM SULFATE, 1 MM DTT, 0.1 M SODIUM CACODYLATE, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 288K |