1U20

Crystal Structure of Xenopus laevis nudix hydrolase nuclear SnoRNA decapping Protein X29

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-02-20
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.188, 82.990, 111.063
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.900 - 2.100
R-factor	0.20881
Rwork	0.204
R-free	0.25421
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	1.108
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.800	2.150
High resolution limit [Å]	2.100	2.100
Number of reflections	27224
<I/σ(I)>	7.1	2.1
Completeness [%]		99.1
Redundancy	6.8	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.68	295	0.025 M HEPES, 3.75% PEG 6000, pH 7.68, VAPOR DIFFUSION, SITTING DROP, temperature 295K