1U1X
Structure and function of phenazine-biosynthesis protein PhzF from Pseudomonas fluorescens 2-79
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-05-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.600, 99.860, 57.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.490 - 1.880 |
| R-factor | 0.1525 |
| Rwork | 0.150 |
| R-free | 0.19711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1v |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.885 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 43151 | |
| <I/σ(I)> | 14 | 5.4 |
| Completeness [%] | 97.8 | 95.7 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 293 | 0.1M Na-Citrate, 0.2M NH4-Acetate, 28% (w/v) PEG 4000, crystals induced with 10mM 3-hydroxyanthranilic acid (inhibitor), then cross-soaked in mother liquor containing 10mM HHA for 10 days, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
