1U1W
Structure and function of phenazine-biosynthesis protein PhzF from Pseudomonas fluorescens 2-79
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.00133 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.600, 99.860, 57.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.350 |
| R-factor | 0.12005 |
| Rwork | 0.118 |
| R-free | 0.15030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u1v |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.058 |
| Data reduction software | XD |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 116176 | |
| <I/σ(I)> | 11.3 | 3.1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | preincubation with ligand, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
