1U1W
Structure and function of phenazine-biosynthesis protein PhzF from Pseudomonas fluorescens 2-79
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00133 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 92.600, 99.860, 57.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.730 - 1.350 |
R-factor | 0.12005 |
Rwork | 0.118 |
R-free | 0.15030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u1v |
RMSD bond length | 0.026 |
RMSD bond angle | 2.058 |
Data reduction software | XD |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.450 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 116176 | |
<I/σ(I)> | 11.3 | 3.1 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 4 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | preincubation with ligand, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |