1U1U
A. thaliana cobalamine independent methionine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.169, 123.169, 132.902 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.170 - 2.950 |
| R-factor | 0.196 |
| Rwork | 0.191 |
| R-free | 0.30600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u1h |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Number of reflections | 24147 | |
| <I/σ(I)> | 6.07 | 1.81 |
| Completeness [%] | 99.4 | 96.7 |
| Redundancy | 3.508 | 3.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 0.1 M TRIS HCL PH 8.0, 0.2 M PEG 4000, 0.2 M LITHIUM SULFATE, pH 8.00, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
