1U1T
Hfq protein from Pseudomonas aeruginosa. High-salt crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 |
Synchrotron site | SPring-8 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-11-24 |
Detector | RIGAKU RAXIS |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.440, 72.350, 109.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.960 - 1.900 |
R-factor | 0.205 |
Rwork | 0.205 |
R-free | 0.25300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.084 | 0.285 |
Number of reflections | 40524 | |
<I/σ(I)> | 5 | 4.8 |
Completeness [%] | 96.9 | 96.5 |
Redundancy | 6 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 1 M Li2SO4, 0.6 M ammonium sulfate, 100 mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |