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1U1S

Hfq protein from Pseudomonas aeruginosa. Low-salt crystals

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsMPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron siteMPG/DESY, HAMBURG
BeamlineBW6
Temperature [K]100
Detector technologyCCD
Collection date2003-12-06
DetectorMARRESEARCH
Wavelength(s)0.97
Spacegroup nameP 21 21 21
Unit cell lengths61.000, 73.260, 106.180
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution8.000 - 1.600
R-factor0.223
Rwork0.223
R-free0.25000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1hk9
RMSD bond length0.006
RMSD bond angle1.300
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwareAMoRE
Refinement softwareCNS (1.1)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]15.0001.700
High resolution limit [Å]1.6001.600
Rmerge0.0420.325
Number of reflections61968
<I/σ(I)>15.93.7
Completeness [%]97.193.8
Redundancy43.5
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP8.5295200 mM NH4Cl, 12% PEG 4000, 50 mM Tris-HCl, 5 mM CdCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K

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