1U1S
Hfq protein from Pseudomonas aeruginosa. Low-salt crystals
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-06 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.000, 73.260, 106.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.600 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hk9 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.042 | 0.325 |
Number of reflections | 61968 | |
<I/σ(I)> | 15.9 | 3.7 |
Completeness [%] | 97.1 | 93.8 |
Redundancy | 4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 200 mM NH4Cl, 12% PEG 4000, 50 mM Tris-HCl, 5 mM CdCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |