1U1Q
Crystal Structure of UP1 Complexed With d(TTAGGGTTA(DI)GG); A Human Telomeric Repeat Containing Inosine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2004-02-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9764 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 50.502, 50.502, 172.250 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.810 - 1.800 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.25800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2up1 |
RMSD bond length | 0.027 |
RMSD bond angle | 2.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.032 | 0.119 |
Number of reflections | 21024 | |
<I/σ(I)> | 46.5 | 15.3 |
Completeness [%] | 97.3 | 92.1 |
Redundancy | 6.17 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 283 | ammonium phosphate, glycerol, Tris, sodium chloride, MES, EDTA, beta-mercaptoethanol , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium phosphate | ||
10 | 1 | 2 | sodium chloride | ||
11 | 1 | 2 | MES | ||
12 | 1 | 2 | EDTA | ||
13 | 1 | 2 | beta-mercaptoethanol | ||
14 | 1 | 2 | H2O | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | EDTA | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | ammonium phosphate | ||
8 | 1 | 2 | glycerol | ||
9 | 1 | 2 | Tris |