1U1P
Crystal Structure of UP1 Complexed With d(TTAGGGTTA 2PR GG); A Human Telomeric Repeat Containing 2-aminopurine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 103 |
Detector technology | IMAGE PLATE |
Collection date | 2002-09-30 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.701, 51.701, 171.098 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 1.900 |
R-factor | 0.237 |
Rwork | 0.237 |
R-free | 0.28100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2up1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.980 |
High resolution limit [Å] | 1.900 | 1.880 |
Rmerge | 0.056 | 0.261 |
Number of reflections | 18967 | |
<I/σ(I)> | 7.8 | 1.8 |
Completeness [%] | 98.8 | 74.6 |
Redundancy | 2.2 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 283 | ammonium phosphate, glycerol, Tris, sodium chloride, MES, EDTA, beta-mercaptoethanol, pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ammonium phosphate | ||
10 | 1 | 2 | sodium chloride | ||
11 | 1 | 2 | MES | ||
12 | 1 | 2 | EDTA | ||
13 | 1 | 2 | beta-mercaptoethanol | ||
14 | 1 | 2 | H2O | ||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | EDTA | ||
6 | 1 | 1 | H2O | ||
7 | 1 | 2 | ammonium phosphate | ||
8 | 1 | 2 | glycerol | ||
9 | 1 | 2 | Tris |