1U1L
Crystal Structure of UP1 Complexed With d(TTAGGGTT PRN GGG); A Human Telomeric Repeat Containing nebularine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-10-28 |
| Detector | RIGAKU JUPITER |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 50.853, 50.853, 172.962 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2up1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.470 |
| Number of reflections | 14752 | |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 91.2 | 87.1 |
| Redundancy | 2.91 | 2.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 283 | ammonium phosphate, glycerol, Tris, sodium chloride, MES, EDTA, beta-mercaptoethanol , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ammonium phosphate | ||
| 10 | 1 | 2 | MES | ||
| 11 | 1 | 2 | EDTA | ||
| 12 | 1 | 2 | glycerol | ||
| 13 | 1 | 2 | beta-mercaptoethanol | ||
| 14 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | sodium chloride | ||
| 4 | 1 | 1 | MES | ||
| 5 | 1 | 1 | EDTA | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | ammonium phosphate | ||
| 8 | 1 | 2 | Tris | ||
| 9 | 1 | 2 | sodium chloride |
