1U1K
Crystal Structure of UP1 Complexed With d(TTAGGGTT 7DA GGG); A Human Telomeric Repeat Containing 7-deaza-adenine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2001-11-08 |
Detector | RIGAKU JUPITER |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.797, 51.797, 171.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.770 - 2.000 |
R-factor | 0.234 |
Rwork | 0.234 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2up1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.072 | 0.317 |
Number of reflections | 14720 | |
<I/σ(I)> | 9.2 | 2.3 |
Completeness [%] | 88.8 | 74.3 |
Redundancy | 2.93 | 2.53 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 283 | PEG 1550, Tris, sodium chloride, MES, EDTA, beta-mercaptoethanol , pH 8.1, VAPOR DIFFUSION, HANGING DROP, temperature 283.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 1550 | ||
10 | 1 | 2 | MES | ||
11 | 1 | 2 | EDTA | ||
12 | 1 | 2 | beta-mercaptoethanol | ||
13 | 1 | H20 | |||
2 | 1 | 1 | Tris | ||
3 | 1 | 1 | sodium chloride | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | EDTA | ||
6 | 1 | 1 | H20 | ||
7 | 1 | 2 | PEG 1550 | ||
8 | 1 | 2 | Tris | ||
9 | 1 | 2 | sodium chloride |