1U1J
A. thaliana cobalamine independent methionine synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-15 |
| Detector | ADSC |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 65 |
| Unit cell lengths | 123.465, 123.465, 132.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.630 - 2.400 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.27500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1u1h |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 44622 | |
| <I/σ(I)> | 12.25 | 2.37 |
| Completeness [%] | 99.0 | 97.8 |
| Redundancy | 5.97 | 5.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 288 | 30% (W/V) POLY-ETHYLENE GLYCOL (PEG) 8000, 0.2 M AMMONIUM SULFATE, 1 MM DTT AND 0.1 M SODIUM CACODYLATE, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
