1U1I
Myo-inositol phosphate synthase mIPS from A. fulgidus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-23 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.770, 83.940, 91.790 |
| Unit cell angles | 65.79, 72.43, 74.98 |
Refinement procedure
| Resolution | 37.600 - 1.900 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jkf |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.018 |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.048 | 0.290 |
| Number of reflections | 92870 | |
| <I/σ(I)> | 13.9 | 1.6 |
| Completeness [%] | 70.4 | 36 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | PEG 4000, potassium sulfate, ammonium sulfate, magnesium chloride, potassium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
