1U16
Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM) in complex with sulfate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X8C |
Synchrotron site | NSLS |
Beamline | X8C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 133.280, 133.280, 73.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.160 - 2.200 |
Rwork | 0.212 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB code:1HY1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.160 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 34371 | |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | ammonium sulfate, cobalt chloride, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |