1U15
Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-03-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.700, 98.800, 107.400 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 35.190 - 2.500 |
| Rwork | 0.221 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB code 1HY1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.190 | 2.580 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 66502 | |
| Completeness [%] | 98.3 | 98.3 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, PEG 2000 MME, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
