1U11
PurE (N5-carboxyaminoimidazole Ribonucleotide Mutase) from the acidophile Acetobacter aceti
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F2 |
Synchrotron site | CHESS |
Beamline | F2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.976 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 99.250, 99.250, 164.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.550 |
R-factor | 0.189 |
Rwork | 0.189 |
R-free | 0.19900 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.600 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 59415 | |
Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 22% PEG4K, 0.19 M AMMONIUM ACETATE, 90 mM CITRATE, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |