1U0X
Crystal structure of nitrophorin 4 under pressure of xenon (200 psi)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.196, 42.461, 52.990 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.450 |
| R-factor | 0.1447 |
| Rwork | 0.143 |
| R-free | 0.17527 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1d2u |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.808 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.300 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.113 |
| Number of reflections | 26873 | |
| <I/σ(I)> | 26.9 | 6.8 |
| Completeness [%] | 96.8 | 78 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | ammonium phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
