1U0Q

Structure of a Llama VHH domain raised against a carbazole molecule

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2001-04-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.988
Spacegroup name	P 32 2 1
Unit cell lengths	48.235, 48.235, 163.983
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.000 - 1.600
R-factor	0.221
Rwork	0.221
R-free	0.25000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.270
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000	1.700
High resolution limit [Å]	1.600	1.600
Rmerge	0.045	0.114
Number of reflections	28590
<I/σ(I)>	8.3	6.3
Completeness [%]	90.4	63
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	293	PEG 5000 30%, AmSO4 200mM, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K