1U0O
The mouse von Willebrand Factor A1-botrocetin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-01-07 |
Detector | MARRESEARCH |
Wavelength(s) | 1.08 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.338, 73.660, 114.424 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.700 |
R-factor | 0.218 |
Rwork | 0.218 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.089 | 0.284 |
Number of reflections | 12759 | |
<I/σ(I)> | 16.1 | 2.5 |
Completeness [%] | 92.4 | 79.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 5000 MME, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |