1U0K
The structure of a Predicted Epimerase PA4716 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2004-04-19 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.778, 69.477, 94.950 |
| Unit cell angles | 90.00, 101.10, 90.00 |
Refinement procedure
| Resolution | 91.290 - 1.500 |
| R-factor | 0.16827 |
| Rwork | 0.167 |
| R-free | 0.18867 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.193 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.570 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.371 | |
| Number of reflections | 75759 | |
| <I/σ(I)> | 5.74 | |
| Completeness [%] | 98.2 | 97.3 |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | di-Ammonium Citrate, PEG 3350, sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
