1U0G
Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-04-10 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.800, 115.600, 73.200 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.21500 |
| Structure solution method | REFINEMENT |
| Starting model (for MR) | NATIVE STRUCTURE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.052 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.271 |
| Number of reflections | 123637 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 97.5 | 81.8 |
| Redundancy | 4.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 1.9 M ammonium sulphate, 100 mM Tris-HCl, pH 8.5 , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
