1U07
Crystal Structure of the 92-residue C-term. part of TonB with significant structural changes compared to shorter fragments
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | MARRESEARCH |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 22.580, 49.320, 72.220 |
Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.130 |
R-factor | 0.139 |
Rwork | 0.137 |
R-free | 0.18300 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 0.037 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SOLVE |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 1.100 |
High resolution limit [Å] | 1.080 | 1.080 |
Rmerge | 0.072 | 0.519 |
Number of reflections | 65348 | |
<I/σ(I)> | 12.68 | 1.51 |
Completeness [%] | 99.8 | 78.5 |
Redundancy | 6.54 | 1.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100mM imidazole, 1.1M sodium citrate, 100mM NaCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |