1U06
crystal structure of chicken alpha-spectrin SH3 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-17 |
Detector | MARRESEARCH |
Wavelength(s) | 1.006231 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.600, 42.250, 49.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.110 - 1.490 |
R-factor | 0.1757 |
Rwork | 0.175 |
R-free | 0.18850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1shg |
RMSD bond length | 0.012 |
RMSD bond angle | 1.531 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.550 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 11318 | |
<I/σ(I)> | 23.5 | 5.3 |
Completeness [%] | 93.9 | 79.9 |
Redundancy | 6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | ammonium sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |