1U00
HscA substrate binding domain complexed with the IscU recognition peptide ELPPVKIHC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Detector technology | CCD |
| Collection date | 2004-02-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 62.761, 83.340, 128.734 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.710 - 1.950 |
| R-factor | 0.1779 |
| Rwork | 0.174 |
| R-free | 0.21038 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DnaK beta subdomain |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.108 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.063 | 0.358 |
| Number of reflections | 24950 | |
| <I/σ(I)> | 9.4 | 2.1 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | potassium sodium tartrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP |
