1TZY
Crystal Structure of the Core-Histone Octamer to 1.90 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX14.2 |
Synchrotron site | SRS |
Beamline | PX14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-12-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.98 |
Spacegroup name | P 65 |
Unit cell lengths | 158.351, 158.351, 103.576 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.960 - 1.900 |
R-factor | 0.18844 |
Rwork | 0.187 |
R-free | 0.22152 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.923 |
Data reduction software | PXGEN |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.960 | 1.948 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.089 | |
Number of reflections | 109956 | |
<I/σ(I)> | 25.7 | |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.7 | 277 | 2.0M KCL, 1.35M PHOSPHATE, pH 6.7, MICRODIALYSIS, temperature 277K |