1TZV
T. maritima NusB, P3121, Form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-01 |
Detector | MARRESEARCH |
Wavelength(s) | 1.05 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.200, 60.200, 87.724 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.710 - 1.350 |
R-factor | 0.199 |
Rwork | 0.199 |
R-free | 0.21300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1EYV |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.450 |
High resolution limit [Å] | 1.350 | 1.350 |
Number of reflections | 41043 | |
<I/σ(I)> | 38.1 | 3.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 5.4 | 5.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 277 | ammonium acetate, sodium phosphate, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |