1TZI
Crystal Structure of the Fab YADS2 Complexed with h-VEGF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-28 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 96.504, 149.588, 117.421 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.2197 |
Rwork | 0.218 |
R-free | 0.25447 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | The VEGF is from pdb entry 1VPF. The Fab is from an in-house determined structure. |
RMSD bond length | 0.011 |
RMSD bond angle | 1.334 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.076 | 0.400 |
Number of reflections | 20861 | |
<I/σ(I)> | 18.7 | 2.5 |
Completeness [%] | 97.3 | 82.92 |
Redundancy | 4.8 | 83.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 292 | LITHIUM CHLORIDE, PEG 6000, MES, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 292K |