1TZH
Crystal Structure of the Fab YADS1 Complexed with h-VEGF
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-06 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.269, 76.269, 112.497 |
| Unit cell angles | 90.00, 105.77, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.600 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.27105 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | The VEGF from pdb entry 1VPF. The Fab from an in-house determined structure. |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.423 |
| Number of reflections | 41828 | |
| <I/σ(I)> | 16.8 | 2.4 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | PEG 3350, ammonium sulfate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
