1TZE
SIGNAL TRANSDUCTION ADAPTOR GROWTH FACTOR, GRB2 SH2 DOMAIN COMPLEXED WITH PHOSPHOTYROSYL HEPTAPEPTIDE LYS-PRO-PHE-PTYR-VAL-ASN-VAL-NH2 (KFPPYVNC-NH2)
Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1995-10-03 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 50.300, 50.300, 89.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.203 |
Rwork | 0.203 |
RMSD bond length | 0.012 |
RMSD bond angle | 24.926 * |
Data reduction software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.100 * |
Rmerge | 0.050 |
Total number of observations | 45126 * |
Number of reflections | 7237 |
Completeness [%] | 100.0 |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 6.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10-25 (mg/ml) | |
2 | 1 | drop | ammonium phosphate | 1.5 (M) | |
3 | 1 | drop | sodium cacodylate | 50 (mM) |