1TZ9
Crystal Structure of the Putative Mannonate Dehydratase from Enterococcus faecalis, Northeast Structural Genomics Target EfR41
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.340, 89.941, 156.756 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.610 - 2.900 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.29200 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB (then SOLVE/RESOLVE) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.610 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.088 | 0.294 |
| Number of reflections | 29389 | |
| <I/σ(I)> | 12.21 | 4.52 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 10% Isopropanol, 10mM DTT, 20% PEG 4k, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
