1TZ7
Aquifex aeolicus amylomaltase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 156.745, 156.745, 169.161 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.150 |
| R-factor | 0.21608 |
| Rwork | 0.215 |
| R-free | 0.24256 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Thermus thermophilus amylomaltase |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.686 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.727 |
| Number of reflections | 75895 | |
| Completeness [%] | 91.7 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 2-methyl-2,4-pentanediol, ammonium sulfate, Tris/HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
