1TZ0
Crystal Structure of Putative Antibiotic Biosythesis Monooxygenase from Bacillus cereus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-03-20 |
Detector | SBC-2 |
Wavelength(s) | 0.9793, 0.9794, 0.954 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 123.894, 86.080, 42.867 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 37.270 - 1.840 |
R-factor | 0.20962 |
Rwork | 0.204 |
R-free | 0.26073 |
Structure solution method | MAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.333 |
Data reduction software | CrystalClear (D*TRECK (MSC/RIGAKU)) |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.910 |
High resolution limit [Å] | 1.840 | 1.840 |
Rmerge | 0.083 | 0.479 |
Number of reflections | 38958 | |
<I/σ(I)> | 9.3 | 2.3 |
Completeness [%] | 99.4 | 95 |
Redundancy | 6.9 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | Sodium Formate, Sodium Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |