1TYQ

Crystal structure of Arp2/3 complex with bound ATP and calcium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Detector technology	IMAGE PLATE
Collection date	2002-10-10
Detector	MARRESEARCH
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	111.368, 129.392, 199.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.550
R-factor	0.234
Rwork	0.232
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k8k
RMSD bond length	0.007
RMSD bond angle	1.280
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.640
High resolution limit [Å]	2.550	2.550
Number of reflections	72816
<I/σ(I)>	21.4	5
Completeness [%]	79.2	68.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	PEG 8000, potassium thiocyanate, Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K