1TYP

SUBSTRATE INTERACTIONS BETWEEN TRYPANOTHIONE REDUCTASE AND N1-GLUTATHIONYLSPERMIDINE DISULPHIDE AT 0.28-NM RESOLUTION

Experimental procedure

Spacegroup name	P 41
Unit cell lengths	128.800, 128.800, 92.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.148
Rwork	0.148
RMSD bond length	0.015
RMSD bond angle	3.300
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.850 *
High resolution limit [Å]	2.800 *	2.800 *
Rmerge	0.096 *	0.240 *
Number of reflections	35848 *
Completeness [%]	70.0 *	36.9 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme solution	18 (mg/ml)
2	1	drop	potasssium phosphate	0.1 (M)
3	1	drop	ammonium sulfate	50 (%(w/v))
4	1	reservoir	ammonium sulphate	80 (%sat)