1TYJ
Crystal Structure Analysis of type II Cohesin A11 from Bacteroides cellulosolvens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.933 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 37.459, 96.708, 110.411 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.600 |
| R-factor | 0.16956 |
| Rwork | 0.168 |
| R-free | 0.19775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qzn |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.460 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.036 | 0.260 |
| Number of reflections | 26333 | |
| <I/σ(I)> | 19.9 | 3.9 |
| Completeness [%] | 97.6 | 93.5 |
| Redundancy | 4.94 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M Tris hydrochloride, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
