1TYJ
Crystal Structure Analysis of type II Cohesin A11 from Bacteroides cellulosolvens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-06-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.933 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 37.459, 96.708, 110.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.550 - 1.600 |
R-factor | 0.16956 |
Rwork | 0.168 |
R-free | 0.19775 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qzn |
RMSD bond length | 0.015 |
RMSD bond angle | 1.460 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.550 | 1.640 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.036 | 0.260 |
Number of reflections | 26333 | |
<I/σ(I)> | 19.9 | 3.9 |
Completeness [%] | 97.6 | 93.5 |
Redundancy | 4.94 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 0.1M Tris hydrochloride, ammonium sulfate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |