1TXL
Crystal structure of metal-binding protein yodA from E. coli, Pfam DUF149
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12B |
| Synchrotron site | NSLS |
| Beamline | X12B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.240, 65.240, 41.340 |
| Unit cell angles | 90.00, 117.66, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.700 |
| R-factor | 0.2282 |
| Rwork | 0.228 |
| R-free | 0.25220 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.245 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.750 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.058 | 0.137 |
| Number of reflections | 20672 | |
| <I/σ(I)> | 44.6 | |
| Completeness [%] | 96.3 | 73.9 |
| Redundancy | 7.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | PEG 1500, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | PEG 1500, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
