1TXJ
Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi
Experimental procedure
| Experimental method | MAD |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 86.633, 86.633, 55.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.860 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23400 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.043 | 0.314 |
| Number of reflections | 16150 | |
| <I/σ(I)> | 54.1 | 8.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.7 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium citrate, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
