1TWN
Crystal structures of ferrous and ferrous-NO forms of verdoheme in a complex with human heme oxygenase-1: catalytic implications for heme cleavage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 119 |
| Detector technology | CCD |
| Collection date | 2003-03-17 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.607, 54.562, 71.720 |
| Unit cell angles | 90.00, 99.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.2381 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qq8 |
| RMSD bond length | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.105 | 0.099 |
| Number of reflections | 24350 | |
| <I/σ(I)> | 22.2 | 2.2 |
| Completeness [%] | 99.1 | 96.3 |
| Redundancy | 7.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 8 | 301 | NH4SO4, Hepes, NaCl, pH 8.0, SMALL TUBES, temperature 301K |
