1TWM
Interleukin-1 Beta Mutant F146Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-31 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 |
| Unit cell lengths | 54.890, 54.890, 77.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.300 - 2.260 |
| R-factor | 0.1763 |
| Rwork | 0.175 |
| R-free | 0.18990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 77.300 |
| High resolution limit [Å] | 2.260 |
| Number of reflections | 10788 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 29% W/V AMMONIUM SULFATE, 100 mM Tris, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
