1TW9
Glutathione Transferase-2, apo form, from the nematode Heligmosomoides polygyrus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-10-07 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.947 |
Spacegroup name | P 1 |
Unit cell lengths | 72.678, 74.034, 88.573 |
Unit cell angles | 79.09, 80.08, 81.55 |
Refinement procedure
Resolution | 25.000 - 1.710 |
R-factor | 0.18132 |
Rwork | 0.180 |
R-free | 0.23198 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1PD2 (Prostaglandin D synthase) |
RMSD bond length | 0.019 |
RMSD bond angle | 1.798 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BEAST |
Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.067 | 0.394 |
Number of reflections | 184048 | |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 95.9 | 81.4 |
Redundancy | 6.2 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 20K, calcium chloride, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |