1TW9
Glutathione Transferase-2, apo form, from the nematode Heligmosomoides polygyrus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.947 |
| Spacegroup name | P 1 |
| Unit cell lengths | 72.678, 74.034, 88.573 |
| Unit cell angles | 79.09, 80.08, 81.55 |
Refinement procedure
| Resolution | 25.000 - 1.710 |
| R-factor | 0.18132 |
| Rwork | 0.180 |
| R-free | 0.23198 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1PD2 (Prostaglandin D synthase) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.798 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 0.394 |
| Number of reflections | 184048 | |
| <I/σ(I)> | 12.6 | 2 |
| Completeness [%] | 95.9 | 81.4 |
| Redundancy | 6.2 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | PEG 20K, calcium chloride, Tris-HCL, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
