1TVN
Cellulase cel5G from Pseudoalteromonas haloplanktis, A family GH 5-2 enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9559 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 135.400, 78.900, 44.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.760 - 1.410 |
| R-factor | 0.15 |
| Rwork | 0.150 |
| R-free | 0.17700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1egz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.800 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.008 | 1.480 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Number of reflections | 87830 | |
| Completeness [%] | 95.1 | 75.3 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 1.3M tri-sodium citrate dihydrate, 40% (v/v) glycerol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
