1TVN
Cellulase cel5G from Pseudoalteromonas haloplanktis, A family GH 5-2 enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-08-30 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9559 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 135.400, 78.900, 44.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 13.760 - 1.410 |
R-factor | 0.15 |
Rwork | 0.150 |
R-free | 0.17700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1egz |
RMSD bond length | 0.012 |
RMSD bond angle | 1.800 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.008 | 1.480 |
High resolution limit [Å] | 1.410 | 1.410 |
Number of reflections | 87830 | |
Completeness [%] | 95.1 | 75.3 |
Redundancy | 2.4 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 1.3M tri-sodium citrate dihydrate, 40% (v/v) glycerol, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |