1TV4
Crystal structure of the sulfite MtmB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-12-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.82700 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.820, 158.820, 136.333 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.850 - 1.800 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.18700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nth |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.065 | 0.347 |
| Number of reflections | 92028 | |
| <I/σ(I)> | 23.7 | 3.7 |
| Completeness [%] | 98.3 | 95.9 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | NACL, HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
