1TV2
Crystal structure of the hydroxylamine MtmB complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-05-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.99200 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 158.170, 158.170, 136.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.970 - 2.000 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.18300 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nth |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.177 | 0.593 |
| Number of reflections | 67189 | |
| <I/σ(I)> | 11.1 | 2.2 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 6.9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | NACL, HEPES, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
