1TUP
TUMOR SUPPRESSOR P53 COMPLEXED WITH DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 117.500, 67.900, 108.800 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.460 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.200 * |
| Rmerge | 0.057 |
| Total number of observations | 123663 * |
| Number of reflections | 36754 * |
| Completeness [%] | 86.0 * |
| Redundancy | 3.36 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA fragment | 1 (M) | |
| 2 | 1 | drop | BTP | 50 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | DTT | 10 (mM) | |
| 5 | 1 | reservoir | PEG400 | 12-15 (%) | |
| 6 | 1 | reservoir | MES | 100 (mM) | |
| 7 | 1 | reservoir | BTP-HCl | 50 (mM) | |
| 8 | 1 | reservoir | DTT | 10 (mM) |
