1TU3
Crystal Structure of Rab5 complex with Rabaptin5 C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-10-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9186 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.400, 83.500, 144.900 |
| Unit cell angles | 90.00, 102.20, 90.00 |
Refinement procedure
| Resolution | 29.720 - 2.310 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n6h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.370 |
| Number of reflections | 54048 | |
| <I/σ(I)> | 11.3 | 2.3 |
| Completeness [%] | 94.9 | 89.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG3350, ammonium bromide, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
