1TTZ
X-ray structure of Northeast Structural Genomics target protein XcR50 from X. campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97896 |
Spacegroup name | P 65 |
Unit cell lengths | 61.690, 61.690, 39.043 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.710 - 2.110 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.21200 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 12147 | |
<I/σ(I)> | 23.98 | 3.3 |
Completeness [%] | 79.8 | 31.6 |
Redundancy | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Hepes, 0.1M NaCl, 1.6M (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Hepes, 0.1M NaCl, 1.6M (NH4)2SO4, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |