1TTW
Crystal structure of the Yersinia Pestis type III secretion chaperone SycH in complex with a stable fragment of YscM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-11-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98397 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.664, 63.664, 162.263 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.380 |
| R-factor | 0.24541 |
| Rwork | 0.243 |
| R-free | 0.29219 |
| Structure solution method | MIR |
| RMSD bond length | 0.044 |
| RMSD bond angle | 3.518 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.480 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.069 | 0.450 |
| Number of reflections | 7916 | |
| <I/σ(I)> | 26.9 | 4 |
| Completeness [%] | 85.0 | 67.5 |
| Redundancy | 13 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.6 | 298 | 1.8 M ammonium sulphate, 200 mM magnesium acetate, 100 mM CAPSO, 2% dioxane, 1.6 mM CHAPS, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
