1TTR
TRANSTHYRETIN-V/122/I CARDIOMYOPATHIC MUTANT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.230, 85.640, 65.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.158 * |
| Rwork | 0.158 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | WILD TYPE TRANSTHYRETIN |
| RMSD bond length | 0.019 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.072 |
| Number of reflections | 19566 |
| Completeness [%] | 96.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 | pH 5.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 41 (%) | |
| 3 | 1 | reservoir | sodium citrate | 200 (mM) |
